By John N. Abelson(Editor), Melvin I. Simon(Editor), Harry Walter(Editor), Gote Johansson(Editor)
This quantity of tools in Enzymology represents the only real number of separation tools according to aqueous two-phase structures. It comprises approaches for the isolation of proteins, in particular enzymes, nucleic acids, mobilephone membranes, and organelles, in addition to for the separation and research of cells. Key positive factors* Use of affinity partitioning for enzyme purification* Separation and learn of cells* Isolation of plasma membranes* huge scale biotechnical use
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Extra resources for Aqueous Two-Phase Systems
Albertsson, this volume . 5 C. Larsson, M. Sommarin, and S. Widell, this volume . 44 GENERAL METHODOLOGY AND APPARATUS  PEGO PHASES ADD CELLS LET SETTLE MI X S A M P IF" ANALYZE Fro. 1. Diagrammatic presentation of partitioning procedure with cells. PEG, PEG-rich phase; D, dextran-rich phase. A known quantity of cells is added to the phases, which are then mixed and permitted to settle by the clock. At the end of the settling time an aliquot is withdrawn from the top phase, and the cell quantity is determined.
Larsson, this volume . 9 ml. The two-phase system is added to all chambers except those which are to contain the samples. 85 ml). , 3-30 mg protein per ml). When preparing the sample systems it is best to mix all components first except for the sample which is added last. Thus, contact of sample with high concentrations of PEG, which might cause protein precipitation, is avoided. , by evaporating water from the phase and then adding sample to restore its original weight) which is added, together with an appropriate amount of upper phase, in the cavities for the sample systems.
A. Albertsson, Fur. J. Biochem. ] Molecular Weights o f Polymers. Polymers of higher molecular weigh! give rise to phase systems at lower concentrations. In some cases, the molecular weights o f the polymers influence partitioning. An example of such an effect in affinity partitioning is shown in Fig. 4. za Different Kinds o f Polymers. , Dx and PEG) are the amount necessary to achieve phase separation, viscosity of the phases, solubility of biopolymers in their presence, price, etc. Determination of Partitioning In principle, almost any analytical method can be used to determine the concentrations o f partitioned material in the phases.