Carbon Nanotubes: Methods and Protocols by Luís F. F. Neves, Ta-Wei Tsai, Naveen R. Palwai, David E.

By Luís F. F. Neves, Ta-Wei Tsai, Naveen R. Palwai, David E. Martyn, Yongqiang Tan (auth.), Kannan Balasubramanian, Marko Burghard (eds.)

Due to their infrequent blend of excessive chemical balance, unheard of optical and electric houses, excessive surface-to-volume ratio, and excessive element ratio, carbon nanotubes (CNTs) have made a huge effect on fabrics technology, molecular biology, biomedicine, and bioanalytical chemistry. Carbon Nanotubes: tools and Protocols offers trustworthy, constant protocols at the software of CNTs in molecular biology-related fields. those are of significant significance, because the commercially on hand CNTs range in purity, agglomeration country, in addition to size and diameter distribution, all of that have a profound impression at the dispersability and floor houses of the tubes. the quantity comprises special sections on functionalization, toxicity, trafficking, scaffolds, and biosensors, supplied by way of specialist researchers from numerous fields. Written within the hugely profitable Methods in Molecular Biology™ sequence structure, chapters contain introductions to their respective subject matters, lists of the required fabrics and reagents, step by step, quite simply reproducible laboratory protocols, and notes on troubleshooting and averting recognized pitfalls.

Authoritative and state-of-the-art, Carbon Nanotubes: tools and Protocols serves to give a contribution to the fulfillment of universal criteria and is helping researchers to prevent discrepancies in destiny biology-related CNT studies.

Show description

By Luís F. F. Neves, Ta-Wei Tsai, Naveen R. Palwai, David E. Martyn, Yongqiang Tan (auth.), Kannan Balasubramanian, Marko Burghard (eds.)

Due to their infrequent blend of excessive chemical balance, unheard of optical and electric houses, excessive surface-to-volume ratio, and excessive element ratio, carbon nanotubes (CNTs) have made a huge effect on fabrics technology, molecular biology, biomedicine, and bioanalytical chemistry. Carbon Nanotubes: tools and Protocols offers trustworthy, constant protocols at the software of CNTs in molecular biology-related fields. those are of significant significance, because the commercially on hand CNTs range in purity, agglomeration country, in addition to size and diameter distribution, all of that have a profound impression at the dispersability and floor houses of the tubes. the quantity comprises special sections on functionalization, toxicity, trafficking, scaffolds, and biosensors, supplied by way of specialist researchers from numerous fields. Written within the hugely profitable Methods in Molecular Biology™ sequence structure, chapters contain introductions to their respective subject matters, lists of the required fabrics and reagents, step by step, quite simply reproducible laboratory protocols, and notes on troubleshooting and averting recognized pitfalls.

Authoritative and state-of-the-art, Carbon Nanotubes: tools and Protocols serves to give a contribution to the fulfillment of universal criteria and is helping researchers to prevent discrepancies in destiny biology-related CNT studies.

Show description

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Mattson MP, Haddon RC, Rao AM (2001) Molecular functionalization of carbon nanotubes and use as substrates for neuronal growth. J Mol Neurosci 14:175–182 12. Yi CQ, Fong CC, Chen WW, Qi SJ, Tzang CH, Lee ST, Yang MS (2007) Interactions between carbon nanotubes and DNA polymerase and restriction endonucleases. Nanotechnology 18:025102 Effects of Carbon Nanotubes on the Proliferation and Differentiation 13. Cui DX, Tian FR, Ozkan CS, Wang M, Gao HJ (2005) Effect of single wall carbon nanotubes on human HEK293 cells.

Thanks Ohio University Condensed Matter and Surface Sciences program for partial support. Temperature and pH-Responsive “Smart” Carbon Nanotube Dispersions 37 References 1. Kim SN, Rusling JF, Papadimitrakopoulos F (2007) Carbon nanotubes for electronic and electrochemical detection of biomolecules. Adv Mater 19:3214–3228 2. Avouris P, Chen Z, Perebeinos V (2007) Carbon-based electronics. Nat Nanotechnol 2:605–615 3. Snow ES, Perkins FK, Robinson JA (2006) Chemical vapor detection using single-walled carbon nanotubes.

The stacking gel should polymerize within 30 min. Once the stacking gel has set, remove the Gel Cassette Assemblies from the Casting Stand. Prepare the inner chamber and lower the inner chamber into the Mini Tank. Add the running buffer to the inner and lower buffer chambers of the Mini Tank. Carefully remove the comb and use a 2-mL syringe fitted with a 22-gauge needle to wash the wells with running buffer. Wash the cultures with cold PBS and add the cell lysis buffer, then determine the protein concentration.

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