By Natalya L Privorotskaya; William P King; Rashid Bashir; Mark A Shannon; Min-Feng Yu
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Extra info for Microcantilever sensors for biochemical detection and analysis
Numerous two-dimensional experiments have been developed in recent years. These have been described at great length and considerable depth by a number of authors including Derome (1988) and Martin and Zektzer (1988). Most of the commonly used experiments are available as microprograms. Implementation of a program requires some knowledge of the mechanisms involved in the experiment itself to allow parameter optimization. It is assumed that either the natural products chemist or a colleague has some NMR expertize to successfully use 2D-NMR.
Unfortunately, if the molecular ion is unstable, it may fragment so extensively that the molecular ion is mitigated to the point of un detect ability . Such destruction can pose a serious problem because an extremely weak molecular ion peak confuses molecular weight determination. ) have been developed in recent years. These various ionization techniques, many of which are readily available, provide the natural products chemist with a number of analytical options. "Hard" and "soft" ionization techniques are complementary processes: if the latter provides an intense molecular ion peak, the former provides fragmentation patterns that can be invaluable in structure determination (Watson 1985).
The ethyl acetate-soluble extract of Alternaria alternata exhibited phytotoxicity toward spotted knapweed. This extract was chromatographed on Bio-Beads S-X4 (hexane/methylene chloride/ethyl acetate, 4: 3: 1). Twelve fractions eluted: fraction five exhibited selective phytotoxicity, fractions four and six general phytotoxicity, toward spotted knapweed. Size exclusion chromatography of fraction five on Sephadex LH-20 (methylene chloride/tertbutyl methyl ether/isopropanol, 1: 1: 1) yielded crystalline maculosin 1.